LOCUS       40924_49272        3547 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD058, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3547)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3547)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    complement(408..428)
                     /note="G5C5; GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons; synthetic"
     misc_feature    429..434
                     /label=XbaI
                     /note="XbaI"
     terminator      complement(442..469)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    478..502
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(522..1238)
                     /label=mEGFP
                     /note="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     misc_feature    complement(1242..1262)
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    1260..1265
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    1284..1289
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(1326..1342)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1350..1366)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1374..1404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1419..1440)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1728..2316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2490..3347)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3348..3452)
                     /label=AmpR promoter
     misc_feature    3478..3483
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggatatc cccccggggg
      421 cccccgattc tagaagcttc aaaaaggcca tccgtcagga tggccttctc ctgcaggacc
      481 actttgtaca agaaagctgg gtcggcgcgc ccaccctttt atttgtacaa ttcatccata
      541 ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc tctcttttcg
      601 tttggatctt tggataattt agattgagtg gataagtaat ggttgtctgg taacaagact
      661 ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac agaaccatct
      721 tcaatgttgt gtctaatttt gaagttaact ttgataccat tcttttgttt gtcagccatg
      781 atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat gttaccatct
      841 tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc ttcaaacttg
      901 acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct ttcttgaaca
      961 taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatatgatc tgggtatcta
     1021 gaaaaacatt gaacaccata agttaaagta gtgactaagg ttggccatgg aactggcaat
     1081 ttaccagtag tacaaataaa ttttaaggtc aatttaccgt aagtagcatc accttcacct
     1141 tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac caaaattggg
     1201 acaacaccag tgaataactc ctcacccttc gacatcatgt catccggcgg cggcggcggg
     1261 atatcctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa
     1321 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1381 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1441 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1501 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1561 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1621 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1681 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1741 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1801 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1861 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1921 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1981 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2041 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2101 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2161 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2221 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2281 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2341 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2401 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2461 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2521 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2581 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2641 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2701 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2761 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2821 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2881 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2941 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3001 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3061 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3121 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3181 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3241 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3301 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3361 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3421 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3481 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3541 tttcgtc
//
